This video gives the basic procedure for reconstituting a lyophilized peptide vial and then filtering through a syringe filter to get rid of bioburden for lab use. A 0.22 µm syringe filter is primarily designed to remove particulates, including bacteria, from solutions.

When used with a reconstituted vial of peptide, this filter will - Remove Bacterial Contamination: The 0.22 µm pore size is small enough to filter out most bacteria, which typically range from 0.5 to 2.0 µm in size. The filter will also remove any particulate matter, such as dust or fibers, that may have been introduced during reconstitution or handling Note, however, it will not remove viruses, endotoxins, or dissolved substances as these are smaller than .22µm.
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All of the reconstitution, filtering and other lab supplies you need can be found at our Lab Supplies Store - www.peptidetest.com/shop

Syringe Filters:
• .22um 4mm PES Individually wrapped filters - www.peptidetest.com/lab-supplies
• .22um 13mm PES Individually wrapped filters - a.co/d/fSBeb6U

Syringe filters are available in a wide variety of configurations but only a few are good for lab use filtering.

• Membrane Material – Only get PES (Polyethersulfone)membranes. They are hydrophilic, have the best flow rate and the lowest protein binding (meaning they won’t absorb the peptide)

• Filter housing size – this is the size of the disc that the filter is in. Common sizes are 4mm, 13mm, 25mm and 33mm.

You want to use the smallest possible filter as a lot of peptide gets left in the filter when you are done using it. For a few approximate examples, a 4mm filter retains about .07ml of reconstituted peptide, a 13mm retains 4x more than a 4mm at about .28ml and a 33mm filter holds a whopping .65ml.

Realistically for individuals filter reconstituted peptides you should use a 4mm filter if you are filtering less than 4ml of peptide or a 13mm filter if you are using more than that.

• Only get individually wrapped sterile filters
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Other Materials
• 70% Isopropyl Alcohol and spray bottles and exam gloves are ubiquitous and can be purchased at any pharmacy or big box retailer like Target or Walmart. Don’t get anything except 70%, as higher percentage blends can evaporate too quicky to kill microbes. Wipes are convenient but you go through a lot cleaning your workspace and gloves.

• Luer lock syringes and needles - depending on your needs 1ml, 3ml, 5ml and 10ml are common sizes. For individual vials reconstituting with between 1-2ml, the 3ml size works great. For needles a good rule of thumb is to stay thinner than 21 gauge so you don’t core a hole in your injection port on your vial. (the numbers go up as the needles get thinner so a 30 gauge is thinner than a 21 gauge)


• Sterile Glass Vials – I use 2ml vials most often but if you are reconstituting a lot, they are available as large as you need.

• Bacteriostatic Water – DO NOT GET ON AMAZON due to past pH and sterility issues. ONLY use Pfizer Hospira brand.

• Sharps Container – The one I use in the video is improvised from a sturdy supplement container that I use in my home lab, I transfer them later to a container made for sharps. You can buy them at drug - www.novocare.com/diabetes/resources/safe-disposal.…